We will be attending CAN-ACN2026 in Montréal (May 18th to 21st). Stop by our booth and come chat with us.

If you are eligible for discounts, you will see them once you are logged in.

MSDS for Lenti viral vectors

Lentiviruses are members of the family Retroviridae. They are enveloped viruses with a singlestranded linear RNA genome. Lentiviruses vector systems can include human viruses (e.g., human immunodeficiency virus). They can also include viruses of animal origin (e.g., feline
immunodeficiency virus, equine infectious anemia virus, simian immunodeficiency virus).

Download PDF

  • PATHOGENICITY

    Lentiviruses can cause severe immunologic and neurologic disease in their natural hosts. The clinical manifestations of infection include non-specific symptoms such as lymphadenopathy, anorexia, chronic diarrhea, weight loss, fever and fatigue.

  • MODE OF TRANSMISSION

    Lentiviruses are transmitted by direct contact with non-intact skin and mucosa and by parenteral inoculation. The hazard of aerosol exposure is unknown.

  • LABORATORY CONTAINMENT REQUIREMENTS

    Containment level 2 or 2+ in some cases.

  • ANIMAL RESEARCH FACILITY CONTAINMENT REQUIREMENTS

    • Recombinant lentiviral vector inoculation : containment level 2.
    • Housing of animals infected with replication defective recombinant lentiviral vector :
      containment level 2 for 7 days. After this period, animals can be transferred to
      containment level 1.
    • Housing of animals permissive for recombinant lentiviral vector replication : containment
      level 2 for duration of experiment.

  • DISINFECTANTS

    0.5% sodium hypochlorite (1/10 dilution of bleach), 70% ethanol, 2% glutaraldehyde and 4% formaldehyde.

  • PHYSICAL INACTIVATION

    Autoclave : 121°C, 30 minutes, 15 psi.

  • ADDITIONAL INFORMATION

    • « Laboratory Biosafety Guidelines », Public Health Agency of Canada.
    • « Containment Standards for Veterinary Facilities », Canadian Food Inspection Agency.
    • « Guidelines for Research Involving Recombinant DNA Molecules », National Institutes of
      Health.
    • « Guidance on Biosafety Considerations for Research with Lentiviral Vectors », National Institutes of Health.
    • Web site of the Comité universitaire de gestion des risques biologiques.

Add address