MSDS for Adeno-associated viral vectors
AAV are members of the family Parvoviridae. They are nonenveloped viruses with a singlestranded linear DNA genome. AAV can only replicate in the presence of a helper virus (e.g., adenovirus, herpes simplex virus, cytomegalovirus, vaccinia virus).
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PATHOGENICITY
There are no known health hazards associated with AAV and they are not known to cause disease in humans.
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MODE OF TRANSMISSION
AAV are transmitted by inhalation of aerosolized droplets, by contact with mucosa, by parenteral injection and by ingestion.
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LABORATORY CONTAINMENT REQUIREMENTS
- Recombinant AAV vector : containment level 1.
- Recombinant AAV vector in the presence of a helper virus : containment level 2.
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ANIMAL RESEARCH FACILITY CONTAINMENT REQUIREMENTS
- Housing of animals infected with recombinant AAV vector : containment level 1.
- Housing of animals infected with recombinant AAV vector in the presence of a helper virus and/or the gene of interest coding for a toxin or an oncogene : containment level 2.
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DISINFECTANTS
- 0.5% sodium hypochlorite (1/10 dilution of bleach), 2% glutaraldehyde and 0.25% sodium dodecyl sulfate.
- Alcohols (ethyl and isopropyl) not effective.
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PHYSICAL INACTIVATION
Autoclave : 121°C, 30 minutes, 15 psi.
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ADDITIONAL INFORMATION
- « Laboratory Biosafety Guidelines », Public Health Agency of Canada.
- « Containment Standards for Veterinary Facilities », Canadian Food Inspection Agency.
- « Guidelines for Research Involving Recombinant DNA Molecules », National Institutes of
Health. - « Guidance on Biosafety Considerations for Research with Lentiviral Vectors », National Institutes of Health.
- Web site of the Comité universitaire de gestion des risques biologiques.
